CLMO engineers and chemists posted the experience in High Performance Liquid Chromatography (HPLC), especially in the on-line SPE-HPLC configuration, where allow direct injection of the high matrix samples, where the sample clean-up by in-line SPE mechanism within the on-line SPE-HPLC system, then follow by the HPLC separation, this will speed up the analysis time, and minimize the human error in off-line sample preparation; among the developed method as follow: